oxbs treatment combined with (Pyrosequencing Inc)
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Oxbs Treatment Combined With, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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1) Product Images from "Exploration of hydroxymethylation in Kagami-Ogata syndrome caused by hypermethylation of imprinting control regions"
Article Title: Exploration of hydroxymethylation in Kagami-Ogata syndrome caused by hypermethylation of imprinting control regions
Journal: Clinical Epigenetics
doi: 10.1186/s13148-015-0124-y
Figure Legend Snippet: Methylation/hydroxymethylation analysis by BS and oxBS treatment at the IG-DMR. a Methylation values (%methylation) determined by pyrosequencing with BS or oxBS conversion for three KOS14 blood samples (Pt1–Pt3), one control pooled blood sample, and one control adult brain sample. Solid lines denote values with BS, whereas dotted lines denote those with oxBS. b Methylation profiles determined by cloning-based sequencing with BS or oxBS conversion for one KOS14 blood sample (Pt1), one control blood, and one control adult brain sample. For BS, filled and open circles indicate (methylated + hydroxymethylated) and unmethylated cytosines at the CpG sites, respectively, while for oxBS, filled and open circles indicate methylated and (hydroxymethylated + unmethylated) cytosines at the CpG sites, respectively. The typing data of A/G SNP (rs10133627) are also denoted. Note this control blood sample was derived from a single person, rather than a pooled blood sample
Techniques Used: Methylation, Control, Cloning, Sequencing, Derivative Assay
Figure Legend Snippet: Representative data of BS/oxBS-array. a Density plots of normalized beta values with BS and oxBS conversion for three KOS14 blood samples (Pt1–Pt3), one control pooled blood sample, and one control adult brain sample. Beta values show a bimodal distribution for both BS and oxBS conversion samples. Of note, a left-skewed methylated peak is observed in the oxBS brain sample. b Box and whisker plots of Δ β score (corresponding to 5hmC value) for each sample. c Distribution of probes with Δ β ≥ 0.1 according to genomic features for each sample
Techniques Used: Control, Methylation, Whisker Assay